Producing an Undergraduate Experiment Focusing on HPLC and Fluorimetry Techniques using Alkaloids Ambiently Extracted from Goldenseal Root.
Faculty Sponsor
Trisha Russell, Whitworth University
Research Project Abstract
Goldenseal root is a common herbal supplement that contains alkaloids such as berberine, palmatine, and hydrastinine. Therefore it can be used to design an organic chemistry lab to quantitatively analyze the concentration of these alkaloids that are contained in different goldenseal root pills from different manufactures and the variations within the herbal supplements as well as practice using organic techniques such as running TLC plates and preparing HPLC samples. Berberine, palmatine, and hydrastinine were extracted from the goldenseal root samples using an ultrasonic bath at ambient temperature. The suspension was centrifuged and the supernatant was diluted with methanol and syringe filtered before being analyzed using HPLC techniques. In addition, samples were spotted on a TLC plate and analyzed by fluorimetry. Levels of berberine were established varied between the samples from different manufacturers and were found to be between 2.24 mg and 3.91 mg. In addition, differences in the brands of goldenseal root were evident in the TLC plates of the samples. Sample B (SOLARAY) showed to have the largest amount of the alkaloid berberine than the other two samples which was supported by the numerical values found from the HPLC experiment.
Session Number
PS1
Location
Graves Gym
Abstract Number
PS1-c
Producing an Undergraduate Experiment Focusing on HPLC and Fluorimetry Techniques using Alkaloids Ambiently Extracted from Goldenseal Root.
Graves Gym
Goldenseal root is a common herbal supplement that contains alkaloids such as berberine, palmatine, and hydrastinine. Therefore it can be used to design an organic chemistry lab to quantitatively analyze the concentration of these alkaloids that are contained in different goldenseal root pills from different manufactures and the variations within the herbal supplements as well as practice using organic techniques such as running TLC plates and preparing HPLC samples. Berberine, palmatine, and hydrastinine were extracted from the goldenseal root samples using an ultrasonic bath at ambient temperature. The suspension was centrifuged and the supernatant was diluted with methanol and syringe filtered before being analyzed using HPLC techniques. In addition, samples were spotted on a TLC plate and analyzed by fluorimetry. Levels of berberine were established varied between the samples from different manufacturers and were found to be between 2.24 mg and 3.91 mg. In addition, differences in the brands of goldenseal root were evident in the TLC plates of the samples. Sample B (SOLARAY) showed to have the largest amount of the alkaloid berberine than the other two samples which was supported by the numerical values found from the HPLC experiment.
