Primers and PCR to Obtain Aryl Sulfatase B Recombinant
Faculty Sponsor
Trisha Russell, Whitworth University
Research Project Abstract
Arylsulfatase B (ASB) is an important protein for the breakdown of glycosaminoglycans. The absence or misfolding of ASB is known to lead to the clinical disorder mucopolysaccaridoses (MPS VI). To study possible treatments for MPS VI, a source of ASB is important. Therefore, a HEK293 cell line in a Bovine liver was created to over express ASB. This ASB was then targeted through HEK cell screening where the RNA sequence could be assayed to cDNA. Primers were assessed using Primer-BLAST in order to isolate the correct sequence from the cDNA strand of ASB. Once the forward primer and reverse primer codon was assessed proper PCR conditions could be evaluated. ASB was then isolated and created in the PCR product to find the cause of the mutant ARSB.
Session Number
PS1
Location
Graves Gym
Abstract Number
PS1-b
Primers and PCR to Obtain Aryl Sulfatase B Recombinant
Graves Gym
Arylsulfatase B (ASB) is an important protein for the breakdown of glycosaminoglycans. The absence or misfolding of ASB is known to lead to the clinical disorder mucopolysaccaridoses (MPS VI). To study possible treatments for MPS VI, a source of ASB is important. Therefore, a HEK293 cell line in a Bovine liver was created to over express ASB. This ASB was then targeted through HEK cell screening where the RNA sequence could be assayed to cDNA. Primers were assessed using Primer-BLAST in order to isolate the correct sequence from the cDNA strand of ASB. Once the forward primer and reverse primer codon was assessed proper PCR conditions could be evaluated. ASB was then isolated and created in the PCR product to find the cause of the mutant ARSB.
