Research Project Title

Cloning and Expressing PEPN in Lactobacillus helveticus

Session Type

Poster Presentation

Research Project Abstract

Celiac disease is an autoimmune disorder triggered by gluten, a protein found in wheat. Currently there is no treatment for celiac disease besides a gluten free diet. This research was directed toward creating a potential treatment for celiac disease by examining bacterial peptidase that could break down gluten. Aminopeptidase N (PEPN) is an enzyme that hydrolyzes N terminal amino acids from a peptide chain. It cuts most amino acids, but it is slow acting at proline residues. The goal of this research was to clone PEPN gene from the bacterium Lactobacillus helveticus to construct a recombinant enzyme for gluten digestion studies. The PEPN gene was amplified by PCR and ligated into a pET-14b E. coli expression plasmid. PEPN expression was induced with IPTG. Overall, the research was successful in cloning and expressing PEPN.

Session Number

PS1

Location

HUB Multipurpose Room

Abstract Number

PS1-d

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COinS
 
Apr 28th, 9:15 AM Apr 28th, 10:45 AM

Cloning and Expressing PEPN in Lactobacillus helveticus

HUB Multipurpose Room

Celiac disease is an autoimmune disorder triggered by gluten, a protein found in wheat. Currently there is no treatment for celiac disease besides a gluten free diet. This research was directed toward creating a potential treatment for celiac disease by examining bacterial peptidase that could break down gluten. Aminopeptidase N (PEPN) is an enzyme that hydrolyzes N terminal amino acids from a peptide chain. It cuts most amino acids, but it is slow acting at proline residues. The goal of this research was to clone PEPN gene from the bacterium Lactobacillus helveticus to construct a recombinant enzyme for gluten digestion studies. The PEPN gene was amplified by PCR and ligated into a pET-14b E. coli expression plasmid. PEPN expression was induced with IPTG. Overall, the research was successful in cloning and expressing PEPN.