Research Project Title

Berberine Uptake in HepG2 Cells

Session Type

Poster Presentation

Research Project Abstract

Berberine (BBR) is an isoquinoline plant alkaloid that has several pharmacological properties. This research focused on the mechanisms of BBR in lowering cholesterol levels. Human HepG2 cells were treated with BBR. From fluorescence microscopy, colocalization of BBR was determined in the mitochondria of HepG2 cells. Further treatments of different BBR concentrations in HepG2 cells concluded that BBR concentration of 0.5 ug/mL was sufficient to penetrate through the cell membrane. Additionally, western blots were performed to observe BBR-treated Low-Density Lipoprotein Receptor (LDLR), Annexin II, Low-Density Lipoprotein Receptor-related Protein 1 (LRP1), and Insulin Receptor (IR) over different time points. The results show consistent protein expression for the tested six hours, suggesting BBR treatment does not inhibit these proteins. Longer treatments with BBR are proposed to observe any change in protein expression.

Session Number

PS1

Location

HUB Multipurpose Room

Abstract Number

PS1-l

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COinS
 
Apr 28th, 9:15 AM Apr 28th, 10:45 AM

Berberine Uptake in HepG2 Cells

HUB Multipurpose Room

Berberine (BBR) is an isoquinoline plant alkaloid that has several pharmacological properties. This research focused on the mechanisms of BBR in lowering cholesterol levels. Human HepG2 cells were treated with BBR. From fluorescence microscopy, colocalization of BBR was determined in the mitochondria of HepG2 cells. Further treatments of different BBR concentrations in HepG2 cells concluded that BBR concentration of 0.5 ug/mL was sufficient to penetrate through the cell membrane. Additionally, western blots were performed to observe BBR-treated Low-Density Lipoprotein Receptor (LDLR), Annexin II, Low-Density Lipoprotein Receptor-related Protein 1 (LRP1), and Insulin Receptor (IR) over different time points. The results show consistent protein expression for the tested six hours, suggesting BBR treatment does not inhibit these proteins. Longer treatments with BBR are proposed to observe any change in protein expression.