Knockdown of p27 Down Regulates GATA3 Expression in HEI-OC1 and MEF Cell Lines
Faculty Sponsor
Kevin Measor, Gonzaga University
Research Project Abstract
Hair cell death leads to hearing loss, which is permanent due to the inability of mammals to regenerate hair cells. To regenerate hair cells researchers seek to transdifferentiate supporting cells into new hair cells. Atoh1 is a transcription factor that is necessary during development, but not sufficient but not sufficient to regenerate hair cells in the adult cochlea because it requires the correct background of other co-transcription factors to adequately transcribe its target genes. From previous work in the Zuo lab, we know that knocking down p27 can up regulate GATA3 – a known co-transcription factor for Atoh1– in adult supporting cells, thereby enabling transdifferentiation into immature hair cells. Here we investigated how p27 is affecting GATA3. By knocking down p27 with A2CE in both MEF and HEI-OC1 cell lines, we found that p27 can regulate transcription of GATA3. While these in vitro data not match the in vivo data, they did suggest that p27 may regulate GATA3 expression and might have implications in cancer research.
Session Number
PS2
Location
Graves Gym
Abstract Number
PS2-cc
Knockdown of p27 Down Regulates GATA3 Expression in HEI-OC1 and MEF Cell Lines
Graves Gym
Hair cell death leads to hearing loss, which is permanent due to the inability of mammals to regenerate hair cells. To regenerate hair cells researchers seek to transdifferentiate supporting cells into new hair cells. Atoh1 is a transcription factor that is necessary during development, but not sufficient but not sufficient to regenerate hair cells in the adult cochlea because it requires the correct background of other co-transcription factors to adequately transcribe its target genes. From previous work in the Zuo lab, we know that knocking down p27 can up regulate GATA3 – a known co-transcription factor for Atoh1– in adult supporting cells, thereby enabling transdifferentiation into immature hair cells. Here we investigated how p27 is affecting GATA3. By knocking down p27 with A2CE in both MEF and HEI-OC1 cell lines, we found that p27 can regulate transcription of GATA3. While these in vitro data not match the in vivo data, they did suggest that p27 may regulate GATA3 expression and might have implications in cancer research.
