Optimization of Tissue Microarray Production for Immunohistochemistry
Faculty Sponsor
Kerry Breno, Whitworth University
Research Project Abstract
In immunohistochemistry, high capacity screening of tissues is necessary for time and cost effective research. Tissue microarrays, the compilation of pertinent tissues into a single paraffin block, can greatly increase the efficiency of staining and analyzing tissue samples. Tissue microarrays were created through a variety of modifications to the annealing stage of production. The microarray competency was established after treatment with various stains, most prominently hematoxylin and eosin staining. The creation of the tissue microarrays proved to be successful and the implementation of this method provided a more efficient process to observe tissues.
Session Number
PS2
Location
Graves Gym
Abstract Number
PS2-c
Optimization of Tissue Microarray Production for Immunohistochemistry
Graves Gym
In immunohistochemistry, high capacity screening of tissues is necessary for time and cost effective research. Tissue microarrays, the compilation of pertinent tissues into a single paraffin block, can greatly increase the efficiency of staining and analyzing tissue samples. Tissue microarrays were created through a variety of modifications to the annealing stage of production. The microarray competency was established after treatment with various stains, most prominently hematoxylin and eosin staining. The creation of the tissue microarrays proved to be successful and the implementation of this method provided a more efficient process to observe tissues.
