Submission Title

Optimization of Tissue Microarray Production for Immunohistochemistry

Session Number

PS2

Location

Graves Gym

Abstract Number

PS2-c

Abstract

In immunohistochemistry, high capacity screening of tissues is necessary for time and cost effective research. Tissue microarrays, the compilation of pertinent tissues into a single paraffin block, can greatly increase the efficiency of staining and analyzing tissue samples. Tissue microarrays were created through a variety of modifications to the annealing stage of production. The microarray competency was established after treatment with various stains, most prominently hematoxylin and eosin staining. The creation of the tissue microarrays proved to be successful and the implementation of this method provided a more efficient process to observe tissues.

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Apr 23rd, 1:30 PM Apr 23rd, 3:00 PM

Optimization of Tissue Microarray Production for Immunohistochemistry

Graves Gym

In immunohistochemistry, high capacity screening of tissues is necessary for time and cost effective research. Tissue microarrays, the compilation of pertinent tissues into a single paraffin block, can greatly increase the efficiency of staining and analyzing tissue samples. Tissue microarrays were created through a variety of modifications to the annealing stage of production. The microarray competency was established after treatment with various stains, most prominently hematoxylin and eosin staining. The creation of the tissue microarrays proved to be successful and the implementation of this method provided a more efficient process to observe tissues.