Submission Title

DNA Sequence Analysis of a Pseudomonas fluorescens Genomic Library

Session Number

PS2

Location

Graves Gym

Abstract Number

PS2-aa

Abstract

Gaeumannomyces graminis var. tritici is a soil-borne fungus that affects the roots of wheat crops. Commonly called “take-all,” this disease causes the yield of wheat crops to drop between 10% and 50%. However, Pseudomonas flourescens bacteria, common in the soil and water, produce antibiotics that inhibit the growth of the fungus and are able to control the disease. We hope to find a biological control solution intertwined in the genetics of Pseudomonas flourescens L5.1-96, a strain known to colonize wheat exceptionally well and fight “take-all” disease. DNA sequence analysis was performed on 24 different DNA fragments from a P. fluorescens L5.1-96 genomic library to elucidate genes contributing to the exceptional ability of this particular strain to super-colonize the wheat and control “take-all.” Among the sequences analyzed, the function of one gene is likely involved in protocatechuate metabolism and the function of another may involve seed storage proteins.

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COinS
 
Apr 23rd, 1:30 PM Apr 23rd, 3:00 PM

DNA Sequence Analysis of a Pseudomonas fluorescens Genomic Library

Graves Gym

Gaeumannomyces graminis var. tritici is a soil-borne fungus that affects the roots of wheat crops. Commonly called “take-all,” this disease causes the yield of wheat crops to drop between 10% and 50%. However, Pseudomonas flourescens bacteria, common in the soil and water, produce antibiotics that inhibit the growth of the fungus and are able to control the disease. We hope to find a biological control solution intertwined in the genetics of Pseudomonas flourescens L5.1-96, a strain known to colonize wheat exceptionally well and fight “take-all” disease. DNA sequence analysis was performed on 24 different DNA fragments from a P. fluorescens L5.1-96 genomic library to elucidate genes contributing to the exceptional ability of this particular strain to super-colonize the wheat and control “take-all.” Among the sequences analyzed, the function of one gene is likely involved in protocatechuate metabolism and the function of another may involve seed storage proteins.